鸡Heme oxygenase 1基因克隆、结构功能预测及组织表达特性分析

doi:10.11843/j.issn.0366-6964.2014.01.005

Abstract

Abstract:

The aim of this study was to clone the chicken Heme oxygenase 1 (HO-1) gene，predict its protein structure and function，and reveal its expression characteristics in tissues.In this study，Hailan White fowl (W-36) was used as experimental animal，the CDS region of chicken HO-1 gene was cloned by qRT-PCR，the protein structure and function were predicted by using bioinformatics，RT-PCR was used to analyze the expression characteristics in tissues.These results showed that the CDS of chicken HO-1 gene was 891 bp，encoding 296 amino acids.The conservation of its nucleotide and amino acid sequences were not high compared with other species.The protein of chicken HO-1 was transmembrane and non-secretory，it had a domain of the HO family，it is classified in EC1.14.99.3.It could bind to HEM，BLA，OXN，Q80，SUC，TRE and Na⁺ molecules，the HO-1 was expressed widely in tissues，and the expression level was higher in the brain，brain stem，artery，sciatic nerve，duodenum and small intestine.HO-1 protein is predicted that it can regulate the sugar and salt function，and is expressed widely in tissues，with important biological function in the circulatory system，nervous system and digestive system.

CLC Number:

JIANG Xiu-qing，WANG Li-li，CAO Chang-yu，LI Nan，GE Yan-song，ZHANG Ying，LI Jin-long. Gene Cloning，Analysis of Bioinformatics and Tissue Specific Expression Profile of Heme Oxygenase 1 Gene in Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(1): 31-37.

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Gene Cloning，Analysis of Bioinformatics and Tissue Specific Expression Profile of Heme Oxygenase 1 Gene in Chicken

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